This page http://phy.ucsf.edu/~idl/colocalization.htm has links for downloading software written by Michael Silver to perform an analysis of the colocalization of labeled structures in serial optical microscopic sections. This method is described in detail in Silver, M.A., and Stryker, M.P. (2000) A method for measuring colocalization of presynaptic markers with anatomically labeled axons using double label immunofluorescence and confocal microscopy. J Neurosci Methods 94: 205-15 See also Erratum .

The self-extracting executable file for Macintosh (Power PC or later) contains 3 files: (1) a customized version of NIH Image (modified from the original version 1.61 written by Wayne Rasband), (2) a macros file that carries out colocalization analysis, and (3) a Microsoft WORD rich text format (rtf) file describing how to use the program. The plain text description of the program is also available for viewing.

To download the entire package, click here ftp://phy.ucsf.edu/pub/stryker_lab/ImageColoc.bin

To view plain text description, click here . ftp://phy.ucsf.edu/pub/stryker_lab/ColocReadme.txt